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Merck KGaA kit milliplex map mouse high sensitivity t-cell magnetic bead panel cat. mhstcmag-70pk
Kit Milliplex Map Mouse High Sensitivity T Cell Magnetic Bead Panel Cat. Mhstcmag 70pk, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cytometric bead array mouse/rat soluble protein master buffer kit
Cytometric Bead Array Mouse/Rat Soluble Protein Master Buffer Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA mouse metabolic hormone magnetic bead panel
Mouse Metabolic Hormone Magnetic Bead Panel, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cba mouse inflammation kit
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Cba Mouse Inflammation Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Merck KGaA mouse insulin assays multiplex assay
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Mouse Insulin Assays Multiplex Assay, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Merck KGaA mouse high-sensitivity t cell magnetic bead panel
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Mouse High Sensitivity T Cell Magnetic Bead Panel, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Merck KGaA magnetic bead panel-immunology multiplex assay
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Magnetic Bead Panel Immunology Multiplex Assay, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Meso Scale Diagnostics LLC mouse th1/th2 9-plex assay ultra-sensitive kit
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Mouse Th1/Th2 9 Plex Assay Ultra Sensitive Kit, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA mouse cytokine/chemokine bio-plex assay kit
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Mouse Cytokine/Chemokine Bio Plex Assay Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems assay kit
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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APELEX Inc infrared beams
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
Infrared Beams, supplied by APELEX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Meso Scale Diagnostics LLC v-plex mouse cytokine 19-plex assay
GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a <t>CBA.</t> Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, <t>cytometric</t> bead array; MCP-1, monocyte chemoattractant protein-1.
V Plex Mouse Cytokine 19 Plex Assay, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a CBA. Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, cytometric bead array; MCP-1, monocyte chemoattractant protein-1.

Journal: Advances in Wound Care

Article Title: New Peroxisome Proliferator-Activated Receptor Agonist (GQ-11) Improves Wound Healing in Diabetic Mice

doi: 10.1089/wound.2018.0911

Figure Lengend Snippet: GQ-11 decreases pro-inflammatory cytokine release and increases anti-inflammatory cytokine release by BMDM. (A) Concentrations of IL-6, MCP-1, and TNF-α protein of BMDM supernatant. (B) IL-10 concentration from supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with LPS (100 ng/mL). (C) IL-10 concentration in supernatant of BMDM pretreated for 24 h with vehicle (DMSO 0.01%), pioglitazone (PIO 10 μM), or GQ-11 (10 μM) and challenged with IL-4 (20 ng/mL). Total protein of BMDM supernatant was accessed by flow cytometry, with a CBA. Negative controls [CTRL (−)] are represented by nontreated and nonchallenged cells. Positive controls [CTRL (+)] are represented by nontreated but challenged cells. Data are expressed as the mean ± SD of biological triplicates. Statistical analyses were performed using ANOVA/Tukey's multiple comparison tests. *p < 0.05. CBA, cytometric bead array; MCP-1, monocyte chemoattractant protein-1.

Article Snippet: Levels of cytokines/chemokines secreted by BMDM were measured in cell culture medium by cytometric bead array (CBA) Mouse Inflammation Kit (BD Biosciences, Cat No.552364).

Techniques: Concentration Assay, Flow Cytometry